خلات الليثيوم
خلات ليثيوم مركب كيميائي له الصيغة CH3COOLi ، وهو ملح الليثيوم لحمض الخل.
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| الأسماء | |
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| اسم أيوپاك المفضل
Lithium acetate | |
| Identifiers | |
| رقم CAS | |
3D model (JSmol)
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| ChEBI | |
| ChemSpider | |
| ECHA InfoCard | 100.008.105 
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| رقم EC |
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| KEGG | |
| عناوين مواضيع طبية MeSH | |
PubChem CID
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| رقم RTECS |
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| UNII | |
CompTox Dashboard (EPA)
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| InChI | InChI={{{value}}} |
| SMILES | |
| الخصائص | |
| الصيغة الجزيئية | C2H3LiO2 |
| كتلة مولية | 65.97 g mol-1 |
| المظهر | crystal |
| الكثافة | 1.26 g/cm3 |
| نقطة الانصهار | |
| قابلية الذوبان في الماء | 45.0 g/100 mL[1] |
| القابلية المغناطيسية | −34.0·10−6 cm3/mol |
| المخاطر | |
| خطر رئيسي | toxic |
| صفحة بيانات السلامة | External MSDS |
| NFPA 704 (معيـَّن النار) | |
| الجرعة أو التركيز القاتل (LD, LC): | |
LD50 (الجرعة الوسطى)
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500 mg/kg (oral, mouse) |
ما لم يُذكر غير ذلك، البيانات المعطاة للمواد في حالاتهم العيارية (عند 25 °س [77 °ف]، 100 kPa). | |
 verify (what is  ?)
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| مراجع الجدول | |
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الاستخدامات
Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected when using lithium acetate gels.
Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.
Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.[2]
المصادر
- ^ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. p. 465. ISBN 0-8493-0594-2.
- ^ Norcum, MT (Aug 15, 1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents". The Journal of Biological Chemistry. 266 (23): 15398–405. PMID 1651330.
| أملاح وإستر أيون الخلات | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AcOH | He | ||||||||||||||||||
| LiOAc | Be(OAc)2 BeAcOH |
B(OAc)3 | ROAc | NH4OAc | AcOAc | FAc | Ne | ||||||||||||
| NaOAc | Mg(OAc)2 | Al(OAc)3 ALSOL Al(OAc)2OH |
Si | P | S | ClAc | Ar | ||||||||||||
| KOAc | Ca(OAc)2 | Sc(OAc)3 | Ti(OAc)4 | VO(OAc)3 | Cr(OAc)2 | Mn(OAc)2 MnAc3 |
Fe(OAc)2 FeAc3 |
Co(OAc)2, CoAc3 |
Ni(OAc)2 | Cu(OAc)2 | Zn(OAc)2 | Ga(OAc)3 | Ge | As | Se | BrAc | Kr | ||
| RbOAc | Sr(OAc)2 | Y(OAc)3 | Zr(OAc)4 | Nb | Mo(OAc)2 | Tc | Ru | Rh | Pd(OAc)2 | AgOAc | Cd(OAc)2 | In | Sn(OAc)2 SnAc4 |
Sb(OAc)3 | Te | IAc | Xe | ||
| CsOAc | Ba(OAc)2 | Hf | Ta | W | Re | Os | Ir | Pt(OAc)2 | Au | Hg2(OAc)2, HgAc2 |
TlOAc Tl(OAc)3 |
Pb(OAc)2 Pb(OAc)4 |
Bi(OAc)3 | Po | At | Rn | |||
| Fr | Ra | Rf | Db | Sg | Bh | Hs | Mt | Ds | Rg | Cn | Uut | Fl | Uup | Lv | Uus | Uuo | |||
| ↓ | |||||||||||||||||||
| La(OAc)3 | Ce(OAc)x | Pr | Nd | Pm | Sm(OAc)3 | Eu(OAc)3 | Gd(OAc)3 | Tb | Dy(OAc)3 | Ho(OAc)3 | Er | Tm | Yb(OAc)3 | Lu(OAc)3 | |||||
| Ac | Th | Pa | UO2(OAc)2 | Np | Pu | Am | Cm | Bk | Cf | Es | Fm | Md | No | Lr | |||||